PCCA Knockout cell line (THP-1)

ENCN
location： Home > Products > KO Cell Lines > PCCA Knockout cell line (THP-1)

PCCA Knockout cell line (THP-1) Order

PCCA Knockout cell line (THP-1)
Catalog#
YKO-HT0729
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PCCA Knockout cell line (THP-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PCCA Knockout cell line (THP-1)
Species
Human
Gene Name
PCCA
Gene ID
5095
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript PCCA-202)
Validation: PCR+Sanger Sequencing
PCCA Knockout cell line (THP-1) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PCCA gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PCCA gene knockout PCCA Knockout cell line (THP-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
PCCA Knockout cell line (THP-1)
PCCA Knockout cell line (THP-1)
Catalog#
YKO-HT0729
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • PCCA Knockout cell line(Hep G2)

    PCCA Knockout cell line(Hep G2)
      inquiry   More details

    Organism: Human
    Gene Name: PCCA
    Gene ID: 5095
    Size: 1*10^6
    Catalog#: YKO-H514
    YKO-H514(Hep G2)
  • PCCA Knockout cell line (HCT 116)

    PCCA Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: PCCA
    Gene ID: 5095
    Size: 1*10^6
    Catalog#: YKO-XN91785
    YKO-XN91785(HCT 116)
  • PCCA Knockout cell line (HEK293)

    PCCA Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: PCCA
    Gene ID: 5095
    Size: 1*10^6
    Catalog#: YKO-XN91784
    YKO-XN91784(HEK293)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • PCCA Knockout cell line(Hep G2)

    PCCA Knockout cell line(Hep G2)

    Organism: Human
    Gene Name: PCCA
    Gene ID: 5095
    Size: 1*10^6
    Catalog#: YKO-H514
      inquiry   More details
  • PCCA Knockout cell line (HCT 116)

    PCCA Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: PCCA
    Gene ID: 5095
    Size: 1*10^6
    Catalog#: YKO-XN91785
      inquiry   More details
  • PCCA Knockout cell line (HEK293)

    PCCA Knockout cell line (HEK293)

    Organism: Human
    Gene Name: PCCA
    Gene ID: 5095
    Size: 1*10^6
    Catalog#: YKO-XN91784
      inquiry   More details
×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
WhatsApp: +86 153 6067 3248
TEL: +86 153 6067 3248 (Int'l)
US Toll free: 855 777 3210
EU Toll free: 800 3272 9252
Korea Toll free: 001 800 3272 9252

Gene Editing Services

EZ-editor™ Products

Copyright © 2024 Ubigene. All rights reserved.
×

Search documentation

Literature:

Name: *

Company: *

Telephone: *

Email:

Notes: After submitting the order, we will contact you as soon as possible.

request now